Which of the following methods is used to determine the presence of growth in mycoplasma broth media? A) Broth turbidity B) Presence of biofilm on surface C) Change in phenol red pH indicator D) Release of radiolabeled CO₂

Question

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Change in phenol red pH indicator can be used to determine the presence of growth in mycoplasma broth media. Mycoplasmas are known to metabolize glucose and produce acid, which can cause a decrease in the pH of the media, leading to a color change in the pH indicator. The absence of a color change indicates the absence of mycoplasma growth. The other options (A, B, D) are not commonly used methods for detecting mycoplasma growth in broth media.

Which of the Following Methods Is Used to Determine the Presence