When using the isolation streaking technique, the greatest concentration of organisms (if present) will be in which quadrant?
A) Fourth
B) Third
C) Second
D) First

Question

Quizplus · Accepted Answer

The isolation streaking technique involves spreading a small amount of the original sample on one quadrant of the plate and then using a sterile loop to streak the bacteria in a zigzag pattern into the next quadrant. This process is repeated until the fourth quadrant is reached. As a result, the greatest concentration of organisms will be in the first quadrant, and progressively less in each subsequent quadrant. Therefore, the best choice is D, first quadrant.

When Using the Isolation Streaking Technique, the Greatest Concentration of Organisms