Which research technique necessarily makes use of reverse transcriptase?
A) Gene cloning
B) Construction of a gene library
C) Construction of a cDNA library
D) Gene knockout studies
E) Pharming

Question

Quizplus · Accepted Answer

Construction of a cDNA library involves the use of reverse transcriptase to create complementary DNA (cDNA) from messenger RNA (mRNA). Reverse transcriptase is an enzyme that can produce a DNA strand from an RNA template, which is necessary for creating a cDNA library. The other options may not require the use of reverse transcriptase or may use it in a limited capacity.

Which Research Technique Necessarily Makes Use of Reverse Transcriptase