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Suppose one restriction enzyme (enzyme A)makes two cuts in a linear DNA fragment and another restriction enzyme (enzyme B)makes one cut in that same DNA.The cuts are not in the same place,and none of the cut fragments are exactly the same size.The restriction digest is set up on an electrophoresis gel.
-How many bands should the gel pick up if both enzymes A and B are used in the restriction digest?
A) Two
B) Three
C) Four
D) Five
E) Six

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Quizplus · Accepted Answer

Enzyme A cuts the linear DNA fragment at two different places, which would result in three fragments of different sizes. Enzyme B cuts the same DNA fragment at one place, which would result in two fragments of different sizes. Therefore, when both enzymes A and B are used, there will be a total of four bands on the electrophoresis gel.

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