Question 1

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-_______________ are enzymes that split DNA at specific base-pair sequences.
A)ligase
B)restriction enzymes
C)DNA microarray
D)expression
E)Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Accepted Answer

Restriction enzymes are specifically designed to cut DNA at specific base-pair sequences, allowing for precise manipulation of DNA fragments.

Question 2

The technique in which DNA fragments are separated by electrophoresis and identified using a radiolabeled probe is called _____________________.

Accepted Answer

Southern blotting

Question 3

The enzyme that catalyzes the formation of a phosphodiester linkage at a break in a DNA strand is _____________________.

Accepted Answer

DNA ligase

Question 4

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-The enzyme that joins two DNA molecules is _______________.
A)ligase
B)restriction enzymes
C)DNA microarray
D)expression
E)Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Accepted Answer

Ligase is the enzyme that joins two DNA molecules by catalyzing the formation of a phosphodiester bond between the 3' hydroxyl of one end and the 5' phosphate group of another DNA end.

Question 5

PCR is the abbreviation for _____________________, which is an in vitro technique used to make multiple copies of a DNA molecule.

Accepted Answer

The answer of PCR is the abbreviation for _____________________, which...

Question 6

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-DNA sequencing by controlled termination of replication is called the ______________ method.
A)ligase
B)restriction enzymes
C)DNA microarray
D)expression
E)Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Accepted Answer

The method described is commonly known as the Sanger method, named after its inventor Frederick Sanger.

Question 7

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-_______________is a method for analyzing the expression of thousands of genes.
A)ligase
B)restriction enzymes
C)DNA microarray
D)expression
E)Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Accepted Answer

DNA microarray is the method used for analyzing the expression of thousands of genes. None of the other options are related to this method.

Question 8

Complementary DNA (cDNA) is formed by the action of reverse transcriptase on _____________________.

Accepted Answer

The answer of Complementary DNA (cDNA) is formed by the...

Question 9

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-_______________ is the enzyme used to create DNA from RNA.
A)ligase
B)restriction enzymes
C)DNA microarray
D)expression
E)Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Accepted Answer

Reverse transcriptase is the enzyme used to create DNA from RNA through the process of reverse transcription.

Question 10

_____________________ cleave DNA at sites with inverted repeat sequences referred to as palindromic sequences.

Accepted Answer

The answer of _____________________ cleave DNA at sites with inverted...

Question 11

The Sanger technique for sequencing DNA involves the use of _____________________ nucleotide analogs that terminate chain elongation.

Accepted Answer

The answer of The Sanger technique for sequencing DNA involves...

Question 12

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-A plasmid is an example of a common _______________.
A)ligase
B)restriction enzymes
C)DNA microarray
D)expression
E)Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Accepted Answer

A plasmid is a type of vector commonly used in genetic engineering to transport foreign genetic material into a host cell.

Question 13

Bacterial plasmid DNA and bacteriophage DNA are commonly used _____________________ to introduce foreign DNA into a bacterium.

Accepted Answer

The answer of Bacterial plasmid DNA and bacteriophage DNA are...

Question 14

The enzyme _____________________ can be used to add nucleotides to the 3` end of DNA.

Accepted Answer

The answer of The enzyme _____________________ can be used to...

Question 15

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-Instead of radioactivity, current DNA sequencing commonly uses _______________ base analogues.
A)ligase
B)restriction enzymes
C)DNA microarray
D)expression
E)Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Accepted Answer

Fluorescent base analogues, such as dideoxynucleotides labeled with different fluorescent dyes, are commonly used in current DNA sequencing methods.

Question 16

Complementary, single-strand overhangs that are produced by some restriction endonucleases are referred to as _____________________.

Accepted Answer

The answer of Complementary, single-strand overhangs that are produced by...

Question 17

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-_______________: A collection of DNA sequences representing all of the mRNA expressed in a particular cell type.
A)ligase
B)restriction enzymes
C)DNA microarray
D)expression
E)Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Accepted Answer

cDNA (complementary DNA) is synthesized from mRNA through reverse transcription, representing all of the mRNA expressed in a particular cell type, thus reflecting the genes actively expressed.

Question 18

DNA fragments can be visualized in polyacrylamide-gel electrophoresis by staining with _____________________, which binds to double-stranded DNA and fluoresces an intense orange when irradiated by UV light.

Accepted Answer

The answer of DNA fragments can be visualized in polyacrylamide-gel...

Question 19

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-The type of vector used for synthesis of protein is _______________.
A)ligase
B)restriction enzymes
C)DNA microarray
D)expression
E)Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Accepted Answer

The answer of Use the following to answer questions 
Choose...

Question 20

Use the following to answer questions 
Choose the correct answer from the list below. Not all of the answers will be used.
-DNA sequences are amplified by the _______________.
A)ligase
B)restriction enzymes
C)DNA microarray
D)expression
E)Sanger
F)footprint
G)vector
H)E. coli
I)cDNA
J)reverse trancriptase
K)fluorescent
L)polymerase chain reaction

Accepted Answer

Polymerase chain reaction (PCR) is the technique used to amplify DNA sequences. It is a rapid and efficient way to make millions or billions of copies of a specific DNA sequence.

Quiz 41: Recombinant Dna Techniques

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -_______________ are enzymes that split DNA at specific base-pair sequences.

The technique in which DNA fragments are separated by electrophoresis and identified using a radiolabeled probe is called _____________________.

The enzyme that catalyzes the formation of a phosphodiester linkage at a break in a DNA strand is _____________________.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -The enzyme that joins two DNA molecules is _______________.

PCR is the abbreviation for _____________________, which is an in vitro technique used to make multiple copies of a DNA molecule.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -DNA sequencing by controlled termination of replication is called the ______________ method.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -_______________is a method for analyzing the expression of thousands of genes.

Complementary DNA (cDNA) is formed by the action of reverse transcriptase on _____________________.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -_______________ is the enzyme used to create DNA from RNA.

_____________________ cleave DNA at sites with inverted repeat sequences referred to as palindromic sequences.

The Sanger technique for sequencing DNA involves the use of _____________________ nucleotide analogs that terminate chain elongation.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -A plasmid is an example of a common _______________.

Bacterial plasmid DNA and bacteriophage DNA are commonly used _____________________ to introduce foreign DNA into a bacterium.

The enzyme _____________________ can be used to add nucleotides to the 3` end of DNA.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -Instead of radioactivity, current DNA sequencing commonly uses _______________ base analogues.

Complementary, single-strand overhangs that are produced by some restriction endonucleases are referred to as _____________________.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -_______________: A collection of DNA sequences representing all of the mRNA expressed in a particular cell type.

DNA fragments can be visualized in polyacrylamide-gel electrophoresis by staining with _____________________, which binds to double-stranded DNA and fluoresces an intense orange when irradiated by UV light.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -The type of vector used for synthesis of protein is _______________.

Use the following to answer questions Choose the correct answer from the list below. Not all of the answers will be used. -DNA sequences are amplified by the _______________.