AST was performed using Mueller-Hinton agar with 5% sheep blood,35°C in 5% CO₂ from 24 hours according to standard protocols,what is the most likely identity of the organism being tested?
A)S.aureus
B)Streptococcus pneumoniae
C)Neisseria gonorrhoeae
D)Moraxella catarrhalis

Question

Quizplus · Accepted Answer

Standardized conditions for AST by disk diffusion of Streptococcus spp.require M-H media supplemented with 5% sheep blood and enriched atmosphere for 20 to 24 hours.S.aureus does not require specialized media (although 2% NaCl can be incorporate to aid in the detection of oxacillin-R).Routine susceptibility testing is not usually done on N.gonorrhoeae or M.catarrhalis.If AST is done on N.gonorhoeae,then growth supplements are added and incubated in 5% CO₂.

AST Was Performed Using Mueller-Hinton Agar with 5% Sheep Blood,35°C