Standardization of antimicrobial susceptibility testing optimizes bacterial growth so inhibition of growth can be attributed to the antimicrobial agents tested and results are accurate,reproducible,and consistent regardless of the microbiology laboratory performing the test.Which of the following incubation conditions are used for common (i.e. ,nonfastidious)bacterial isolates using manual techniques?
A)16 to 20 hours,ambient temperature,5% to 7% CO₂
B)16 to 20 hours,35°C,ambient air
C)20 to 24 hours,ambient temperature,5% to 7% CO₂
D)20 to 24 hours,35°C,ambient air

Question

Quizplus · Accepted Answer

Incubation conditions for antimicrobial susceptibility testing (AST)of rapidly growing organisms frequently isolated from clinical samples and known pathogens of human infections include duration of 16 to 20 hours,approximate body temperature of 35°C,and room air.Some more fastidious organisms may require longer incubation times and CO₂ enriched atmosphere,as well supplements added to media used for testing.

Standardization of Antimicrobial Susceptibility Testing Optimizes Bacterial Growth So Inhibition