Which of the following relates to restriction fragment length polymorphism (RFLP) analysis?
A) can be used to detect one-base differences in DNA sequences
B) detects differences in conformation of single strands of DNA
C) requires the use of an RNA intermediate
D) uses Western blotting as part of the technique
E) uses Sanger DNA sequencing as part of the technique

Question

Quizplus · Accepted Answer

RFLP analysis can detect one-base differences in DNA sequences by identifying variations in the length of DNA fragments produced by restriction enzyme digestion.  Pathogenic alterations to the genotypic can be due to deletions or insertions within the gene being analyzed or even single nucleotide substitutions that can create or delete a restriction enzyme recognition site. RFLP analysis takes advantage of this and utilizes Southern blotting of restriction enzyme digested genomic DNA to detect familial patterns (polymorphisms) of the fragments of a given gene, detectable by screening the Southern blot with a probe corresponding to the gene of interest.

Which of the Following Relates to Restriction Fragment Length Polymorphism