In order to generate a genomic library which of the following enzymes is required?
A) DNase I
B) DNA polymerase
C) polynucleotide kinase
D) restriction endonuclease
E) reverse transcriptase

Question

Quizplus · Accepted Answer

Restriction endonucleases are used to cut DNA at specific sequences, which is essential for creating a genomic library by allowing the insertion of DNA fragments into vectors. Genomic DNA can be isolated from any cell or tissue for cloning. The genomic DNA is first digested with restriction enzymes to generate fragments in the size range that are optimal for the vector being utilized for cloning.

In Order to Generate a Genomic Library Which of the Following