The Sanger method of nucleotide sequence determination involves
A) the activity of DNA polymerase
B) the use of chemical agents that modify DNA
C) the transfer of electrophoretically resolved DNA to a nitrocellulose filter.
D) the incorporation of poly dT the 3'-OH end of a DNA strand.

Question

Quizplus · Accepted Answer

The Sanger method of DNA sequencing involves the activity of DNA polymerase in adding fluorescently tagged nucleotides to a growing DNA strand. As each tagged nucleotide is incorporated, the reaction is paused and the resulting fluorescence intensity is measured in order to determine the identity and order of the nucleotides in the DNA sequence.

The Sanger Method of Nucleotide Sequence Determination Involves