X⁻,Y⁻,and Z⁻ are separated with an anion exchange column with a mobile phase containing A⁻.The strength,which the anions bind to the column,is Y⁻ X⁻ Z⁻ A⁻.To elute the anions,the concentration of A⁻ is____________________ ,with____________________ eluting first,____________________ eluting second,and____________________ eluting last. A)decreased;Y⁻;X⁻;Z⁻ B)increased;Z⁻;X⁻;Y⁻ C)decreased;Z⁻;X⁻;Y⁻ D)increased;Y⁻;X⁻;Z⁻ E)increased;X⁻;Y⁻;Z⁻

Question

X⁻,Y⁻,and Z⁻ are separated with an anion exchange column with a mobile phase containing A⁻.The strength,which the anions bind to the column,is Y⁻ > X⁻ > Z⁻ > A⁻.To elute the anions,the concentration of A⁻ is____________________ ,with____________________ eluting first,____________________ eluting second,and____________________ eluting last. A)decreased;Y⁻;X⁻;Z⁻ B)increased;Z⁻;X⁻;Y⁻ C)decreased;Z⁻;X⁻;Y⁻ D)increased;Y⁻;X⁻;Z⁻ E)increased;X⁻;Y⁻;Z⁻

Quizplus · Accepted Answer

To elute the anions from strongest to weakest binding, the concentration of the mobile phase anion (A⁻) must be increased. Z⁻, being the weakest binder, elutes first, followed by X⁻, with Y⁻, the strongest binder, eluting last.

X−,Y−,and Z− Are Separated with an Anion Exchange Column with a Mobile

X⁻,Y⁻,and Z⁻ Are Separated with an Anion Exchange Column with a Mobile