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When Deciding Between an Isocratic Elution or a Gradient Elution,a Δ\Delta

Question 20

Multiple Choice

When deciding between an isocratic elution or a gradient elution,a scouting gradient should be done.Which of the criteria below is INCORRECT?


A) If Δ\Delta t/tG < 0.25,use isocratic elution.
B) If Δ\Delta t/tG > 0.40,use gradient elution.
C) For 0.25 < Δ\Delta t/tG < 0.40 use either an isocratic or gradient elution,the decision depending on factors such as available equipment or complexity of the sample.
D) For Δ\Delta t/tG < 0.25,a good starting isocratic elution solvent composition is the composition halfway through the Δ\Delta t of the scouting gradient.
E) For 0.25 < Δ\Delta t/tG < 0.40,a good starting isocratic elution solvent composition is the composition halfway through the Δ\Delta tG of the scouting gradient.

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