You are studying RNA processing in a cell-free system. You find that incomplete RNA processing is occurring in your system and that the failure is most likely due to incomplete assembly of the splicesome complex. Which of the following RNA-processing events is most likely defective in your assay system?
A) addition of poly(A)
B) guiding ribosomes to the endoplasmic reticulum
C) modification of the 5′ end of hnRNA
D) removal of introns in hnRNA
E) transport of mRNA to the cytoplasm

Question

Quizplus · Accepted Answer

The spliceosome is responsible for the removal of introns from hnRNA (heterogeneous nuclear RNA) during RNA processing. If the spliceosome assembly is incomplete, this splicing process would be defective.  A spliceosome is a complex of small nuclear RNAs (snRNAs) and proteins, which is responsible for the catalytic removal of introns from a transcribed pre-mRNA in the process referred to as RNA splicing

You Are Studying RNA Processing in a Cell-Free System