How is reverse transcriptase used to clone genes?
A) Reverse transcriptase is used to make a cDNA copy of an mRNA strand.
B) Reverse transcriptase is used to make a cloned DNA copy of a plasmid DNA molecule.
C) Reverse transcriptase is necessary to read the information contained on the cDNA molecule.
D) Reverse transcriptase transcribes RNA from cDNA in reverse order.
E) Reverse transcriptase transcribes cDNA into RNA without transcription of introns.

Question

Quizplus · Accepted Answer

Reverse transcriptase is used to synthesize complementary DNA (cDNA) from an mRNA template, which is a crucial step in cloning genes, especially for eukaryotic genes that contain introns.

How Is Reverse Transcriptase Used to Clone Genes