To avoid the introduction of introns into the vector, a ____ copy of mature mRNA is made, using the enzyme ____.
A) sDNA; RNA polymerase
B) cDNA; DNA ligase
C) cDNA; reverse transcriptase
D) sDNA; reverse transcriptase
E) cDNA; DNA polymerase

Question

Quizplus · Accepted Answer

cDNA (complementary DNA) is synthesized from a mature mRNA template using the enzyme reverse transcriptase. This process avoids the inclusion of introns because mature mRNA, which serves as the template, has already had its introns removed during RNA processing.

To Avoid the Introduction of Introns into the Vector, a ____