A cluster of cases of legionellosis have occurred in your neighborhood. People with the illness have cough, fever, shortness of breath, chest pain and sometimes diarrhea. The illness is caused by the bacterium Legionella pneumophila, a facultative intracellular parasite that survives in certain ameba. The organism has an absolute requirement for L-cysteine.
-A modification of the BCYE medium used to culture L. pneumophila is BCYE with DGVP. This medium contains glycine, polymixin B (an antifungal agent), and and vancomycin (antibiotic), as well as two dyes (bromocreson purple and bromthymol blue) that facilitate identification of the organism based on colony color. What type of medium is BCYE with DGVP?
A) Selective
B) Differential
C) Selective AND differential
D) Liquid
E) Anaerobic

Question

A cluster of cases of legionellosis have occurred in your neighborhood. People with the illness have cough, fever, shortness of breath, chest pain and sometimes diarrhea. The illness is caused by the bacterium Legionella pneumophila, a facultative intracellular parasite that survives in certain ameba. The organism has an absolute requirement for L-cysteine.
-A modification of the BCYE medium used to culture L. pneumophila is BCYE with DGVP. This medium contains glycine, polymixin B (an antifungal agent), and and vancomycin (antibiotic), as well as two dyes (bromocreson purple and bromthymol blue) that facilitate identification of the organism based on colony color. What type of medium is BCYE with DGVP?
A) Selective
B) Differential
C) Selective AND differential
D) Liquid
E) Anaerobic

Quizplus · Accepted Answer

BCYE with DGVP is both selective and differential. It contains antibiotics and antifungal agents, making it selective for the growth of Legionella pneumophila, while the dyes allow for differentiation of the organism based on colony color.

A Cluster of Cases of Legionellosis Have Occurred in Your