Question 1

An Hfr cell can initiate chromosome transfer from one E. coli to another.

Accepted Answer

Hfr (high frequency recombination) cells have a chromosomally integrated F factor which allows them to initiate conjugation and transfer chromosomal genes from one E. coli cell to another.

Question 2

Bacteria that are in a particular physiological state to become transformed are called
________.

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Question 3

Compare and contrast bacteriophage lysis and lysogeny.

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Question 4

The "interrupted mating technique" provides a genetic map in Drosophila.

Accepted Answer

The "interrupted mating technique" was used to map genes in bacteria, specifically in experiments with E. coli, not in Drosophila.

Question 5

Explain what is meant by the term heteroduplex in the context of bacterial transformation.

Accepted Answer

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Question 6

Bacteriophages that cannot undergo lysogeny but can infect bacteria are called
________.

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Question 7

What are the roles of the recBCD genes?

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Question 8

What is a bacteriophage?

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Question 9

What experimental observation demonstrated that genes are involved in the integration of foreign DNA into the bacterial chromosome?

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Question 10

Present the general structural features of a plasmid and give an example.

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Question 11

A form of bacterial recombination that involves a viral intermediate is called _.

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Question 12

What is meant by the term cotransformation?

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Question 13

What is the consequence of a mutation in the recA gene in bacteria?

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Question 14

Assume that one counted 67 plaques on a bacterial plate where 0.1 ml of a 10 -5
dilution of phage was added to bacterial culture. What is the initial concentration of the undiluted phage?

Accepted Answer

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Question 15

If two different auxotrophic strains are placed in a liquid medium culture tube, prototrophic strains can sometimes be subsequently recovered. Name several mechanisms by which this is possible.

Accepted Answer

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Question 16

Lysogeny is an important phenomenon in bacteria and phages. Briefly describe lysogeny.

Accepted Answer

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Question 17

In a bacterial cross in which the donor (Hfr) is a+b+ and the recipient strain (F -) is a -b -, it is expected that recombinant bacteria will all be a+b+.

Accepted Answer

While bacterial recombination can occur in an Hfr x F- cross, the resulting recombinants are not always a+b+. The recombination event can occur at different points on the bacterial chromosome, resulting in different combinations of alleles in the recombinant cells. Therefore, it is possible for some recombinants to end up a+b+, but it is not expected that all of them will be.

Question 18

When a bacteriophage genome incorporates itself into the chromosome of the host, that
phage genome is referred to as a(n)

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Question 19

What is a significant difference between a lytic and a lysogenic cycle?

Accepted Answer

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Question 20

What term is used to describe the phenomenon in which a bacteriophage genome incorporates its genome into the chromosome of the host?

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Quiz 8: Genetic Analysis and Mapping in Bacteria and Bactierophages