To produce a mutation in a specific E.coli gene, an engineered fragment of DNA in which sequences from each end of that gene flank an antibiotic resistance gene
A)is transformed into cells and replicates independently of the genomic DNA because it has a centromere.
B)is transformed into cells and maintained as a plasmid.
C)is introduced into cells and transposes into the bacterial gene.
D)is introduced into cells and undergoes homologous recombination with the bacterial gene.

Question

Quizplus · Accepted Answer

The engineered fragment of DNA is designed to undergo homologous recombination with the target gene in the E. coli genome. This process replaces the target gene with the antibiotic resistance gene flanked by sequences homologous to those of the target gene, thereby inducing a mutation.

To Produce a Mutation in a Specific E