Question 1

In bacteria,a unique RNA that binds the A site of the ribosome and directs the addition of approximately 12 amino acids to the nascent peptide when no stop codon is present is called the ________.

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The answer of In bacteria,a unique RNA that binds the...

Question 2

________ degrades mRNAs that contain premature stops.

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The answer of ________ degrades mRNAs that contain premature stops....

Question 3

The ________ allows for flexibility in the codon-anticodon interaction at the third position.

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The answer of The ________ allows for flexibility in the...

Question 4

The sequence of amino acids in a protein is based on the ________ of the mRNA.

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Question 5

During elongation,each newly arriving tRNA binds in the region of the ribosome known as the ________ site.

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Question 6

You discover a pattern of a 20 amino acid sequence at the C-terminus of over 50 proteins in an organism.You hypothesize that this amino acid sequence is a signal sequence for localization into a specific organelle or cell compartment,but you don't know which one.How might you use microscopy to investigate the location of proteins with this unique C-terminus sequence?

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The answer of You discover a pattern of a 20...

Question 7

The anticodon base ________ is able to bind U,C,or A,allowing for wobble in the anticodon region during translation.

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Question 8

________ is an intramolecular post-translational process that allows for self-catalytic removal of specific amino acid sequences.

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Question 9

The ________ are enzymes that link amino acids with their appropriate tRNAs.

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Question 10

The transfer of polypeptides into the ER is called ________ because the movement across the membrane is coupled with translation.

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Question 11

Discuss some of the possible ways in which nonsense mutations can potentially be suppressed in a cell.

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Question 12

Within the endoplasmic reticulum,________ recognizes misfolded or unassembled proteins and transports them back to the cytosol.

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Question 13

You want to clone a eukaryotic gene and express the corresponding protein in yeast.However,the protein typically localizes within mitochondria.How will you perform your gene cloning so that the protein is secreted from the cell,rather than localized within yeast mitochondria?

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The answer of You want to clone a eukaryotic gene...

Question 14

You have cloned a eukaryotic gene,and inserted it into a bacterial system for expression.You discover that the gene product is much longer than should be observed based upon the insert you successfully cloned.What problems would result in a longer cloned sequence? How will you go about investigating your hypothesis? Would your answer change based upon the presence or absence of introns? Why or why not?

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The answer of You have cloned a eukaryotic gene,and inserted...

Question 15

The first amino acid incorporated in bacterial protein synthesis is ________.

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Question 16

In bacteria,it is common to produce polycistronic messages; in eukaryotes it is less common.Describe how polycistronic mRNAs are made and processed in prokaryotes and eukaryotes.How might polycistronic mRNA molecules be an advantage for eukaryotes?

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The answer of In bacteria,it is common to produce polycistronic...

Question 17

________ degrades mRNAs that contain no stop codons.

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The answer of ________ degrades mRNAs that contain no stop...

Quiz 19: Gene Expression: Iiprotein Synthesis and Sorting