The following steps must be performed to make a bacterium produce human protein X. 1- Translation
2- Restriction enzyme
3- Prokaryotic transcription
4- DNA ligase
5- Transformation
6- Eukaryotic transcription
7- Reverse transcription
Which of the following places the steps in the correct order?
A) 6, 7, 2, 4, 5, 3, 1
B) 1, 2, 3, 5, 4, 7, 6
C) 5, 2, 3, 4, 7, 6, 1
D) 6, 7, 2, 3, 4, 5, 1
E) 6, 2, 1, 3, 4, 5, 7

Question

Quizplus · Accepted Answer

First, eukaryotic transcription (6) occurs to produce mRNA, then reverse transcription (7) to synthesize cDNA. Restriction enzymes (2) cut DNA, DNA ligase (4) joins DNA fragments, transformation (5) introduces DNA into bacteria, prokaryotic transcription (3) occurs, and finally, translation (1) produces the protein.

The Following Steps Must Be Performed to Make a Bacterium