Which of the following places the steps in the PCR procedure in the correct order?
1) Incubate at 94°C to denature DNA strands;
2) Incubate at 72°C for DNA synthesis;
3) Incubate at 60°C for primer hybridization.
A) 1, 3, 2
B) 1, 2, 3
C) 3, 2, 1
D) 3; 1; 2
E) 2; 1; 3

Question

Quizplus · Accepted Answer

The first step in the PCR procedure is to denature the DNA strands at 94°C. Next, the primer hybridizes to the single-stranded DNA template at 60°C. Lastly, the DNA polymerase extends from the primer to produce new strands of DNA at 72°C. Therefore, the correct order is 1, 3, 2.

Which of the Following Places the Steps in the PCR