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Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler
Edition 2ISBN: 9780130819086Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler
Edition 2ISBN: 9780130819086 Exercise 5
You wish to express a particular peptide in E. coli using a high-copy-number plasmid. You
have the amino acid sequence for the peptide.
a. Explain the experimental process for generating and selecting the genetically engineered
E. coli using restriction enzymes, ligase, E. coli, plasmid with neomycin resistance, and
the known amino acid sequence.
b. What control elements would you place on the plasmid to regulate expression and to pre-
vent read-through?
have the amino acid sequence for the peptide.
a. Explain the experimental process for generating and selecting the genetically engineered
E. coli using restriction enzymes, ligase, E. coli, plasmid with neomycin resistance, and
the known amino acid sequence.
b. What control elements would you place on the plasmid to regulate expression and to pre-
vent read-through?
Explanation
Verified
Verified
a) When the amino acid sequence is known...
Bioprocess Engineering 2nd Edition by Fikret Kargi,Michael Shuler
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