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Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
Edition 6ISBN: 978-1305577206Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
Edition 6ISBN: 978-1305577206 Exercise 18
Distinguishing the mechanisms of Class I and Class II aldolases
Fructose bisphosphate aldolase in animal muscle is a class I aldolase, which forms a Schiff base intermediate between substrate (for example, fructose-1,6-bisphosphate or dihydroxyacetone phosphate) and a lysine at the active site (see Figure 18.12). The chemical evidence for this intermediate comes from studies with aldolase and the reducing agent sodium borohydride, NaBH4. Incubation of the enzyme with dihydroxyacetone phosphate and NaBH 4 inactivates the enzyme. Interestingly, no inactivation is observed if NaBH 4 is added to the enzyme in the absence of substrate. Write a mechanism that explains these observations and provides evidence for the formation of a Schiff base intermediate in the aldolase reaction.
Fructose bisphosphate aldolase in animal muscle is a class I aldolase, which forms a Schiff base intermediate between substrate (for example, fructose-1,6-bisphosphate or dihydroxyacetone phosphate) and a lysine at the active site (see Figure 18.12). The chemical evidence for this intermediate comes from studies with aldolase and the reducing agent sodium borohydride, NaBH4. Incubation of the enzyme with dihydroxyacetone phosphate and NaBH 4 inactivates the enzyme. Interestingly, no inactivation is observed if NaBH 4 is added to the enzyme in the absence of substrate. Write a mechanism that explains these observations and provides evidence for the formation of a Schiff base intermediate in the aldolase reaction.
Explanation
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A Schiff base intermediate is formed whe...
Biochemistry 6th Edition by Reginald Garrett,Charles Grisham
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