Molecular Biology 5th Edition by Robert Weaver

Exercise 4

You are an MD/PhD developmental biologist (highly trained in techniques of molecular biology) studying the pathogenesis of Type I insulin-dependent diabetes mellitus (IDDM). You have several patients who are predisposed to becoming diabetic, and control subjects who have no family history or predisposition to becoming diabetic, enrolled in a clinical study that will involve the removal of a small section of pancreatic beta cells. You want to analyze the differences in gene expression between cells from these two groups of subjects. Describe the experimental method(s) you would use, and what information you hope to obtain from this study.