Molecular Biology 5th Edition by Robert Weaver

Exercise 2

You have isolated an E. coli mutant with an aminoacyl-tRNA synthetase that causes a tRNA with the anticodon 3-UUC-5 to be charged with asparagine at the elevated temperature of 42°C. What effect would you expect this to have on protein synthesis in these cells at 42°C, and why You then isolate another mutant that suppresses the first mutation, and you trace the second mutation to a tRNA gene. What tRNA would you expect to be altered in the second mutant, and where Predict the nature of this alteration.