Molecular Biology 5th Edition by Robert Weaver

Exercise 1

Describe a toeprint assay involving E. coli ribosomal subunits and a fictious mRNA in a cell-free extract that contains all the factors necessary for translation. What results would you expect to see with 30S ribosomal subunits alone With 50S subunits alone With both subunits and all amino acids except leucine, which is required in the 20 th position of the polypeptide