Genetics: Analysis and Principles 5th Edition by Robert Brooker

Exercise 41

A portion of the coding sequence of a cloned gene is shown here:
5 -GCCCCCGATCTACATCATTACGGCGAT-3
3 -CGGGGGCTAGATGTAGTAATGCCGCTA-5
This portion of the gene encodes a polypeptide with the amino acid sequence alanine-proline-aspartic acid-leucine-histidine-histidine-tyrosine-glycine-aspartic acid. Using the method of site-directed mutagenesis, a researcher wants to change the leucine codon into an arginine codon, with an oligonucleotide that is 19 nucleotides long. What is the sequence of the oligonucleotide that should be made You should designate the 5 and 3 ends of the oligonucleotide in your answer. Note: The mismatch should be in the middle of the oligonucleotide, and a 1-base mismatch is preferable over a 2- or 3-base mismatch. Use the bottom strand as the template strand for this site-directed mutagenesis experiment.