Urinalysis and Body Fluids 6th Edition by Majorie DiLorenzo, Susan Strasinger

Exercise 48

Following an abnormal sperm motility test with a normal sperm count, what additional test might be ordered?

A. Fructose level

B. Zinc level

C. MAR test

D. Eosin-nigrosin stain

Step 1 of 2

Eosin-nigrosin stain:

• Presence of increased immobile sperms in motility test needs further evaluation of sperm vitality.

• After abnormal motility test, sperm vitality test should be performed.

• Sperm vitality or viability may be suspected when low percentage motile sperms are discovered in normal sperm count.

• Sperm vitality is evaluated by mixing the sperm specimen with stain named as “Eosin-nigrosin” which is used to find number of dead cells present in 100 sperm cells using phase-contrast or bright field microscope.

Therefore, “Eosin-nigrosin” is a stain used to test sperm vitality after abnormal sperm motility is seen in normal count of sperms. Hence, the correct answer is option.