Urinalysis and Body Fluids 6th Edition by Majorie DiLorenzo, Susan Strasinger

Exercise 45

The principle of the protein-low reagent pad on the Multistix Pro is the:

A. Binding of albumin to sulphonphthalein dye

B. Immunologic binding of albumin to antibody

C. Reverse protein error of indicators reaction

D. Enzymatic reaction between albumin and dye

Step 1 of 2

Siemens Multistix Pro reagent strip:

These strips are used to detect many substances in urine.

The reagent strip has pad for those substances except Urobilinogen.

• Protein reactions are classified as protein-high and protein-low.

• The principle used in protein-low (albumin) reaction is dye binding method.

Dye binding procedure:

• Albumin reagent strip has an absorbent pad for albumin.

• Bis(3’,3”diiodo-4’,4”-dihydroxy-5’,5”-dinitrophenyl)3,4,5,6-tetrabromosulphonphthalein(DIDNTB)-dye is used for the reaction to analyze albumin.

• The dye has high specificity for albumin.

• It measures 8 to150mg/dL (milligrams per deciliter) of albumin excluding other proteins.

• Paper treated with bis-(heptapropylene glycol) carbonate prevents interference of reaction by alkaline urine which is highly buffered.

• Pale green to aqua blue color is formed in the strip, when albumin binds to sulphonphthalein dye.

• Bloody urine and colored urine elevates false results.

Therefore, principle used in reagent strip Multistix Pro for protein-low is “binding of albumin to sulphonphthalein dye”. Hence, the correct answer is option.