Urinalysis and Body Fluids 6th Edition by Majorie DiLorenzo, Susan Strasinger

Exercise 41

The principle of the protein-high pad on the Multistix Pro reagent strip is the:

A. Diazo reaction

B. Enzymatic dye-binding reaction

C. Protein error of indicators

D. Microalbumin-Micral-Test

Step 1 of 2

Siemens Multistix Pro reagent strips:

These strips are used to detect many substances in urine

• The reagent strip has pad for those substances except urobilinogen.

• The Multistix Pro reagent strip have pad for protein-high.

• Protein reactions are classified as protein-high and protein-low.

• The principle used in protein-high reaction is “protein error of indicators”.

Protein error of indicators:

It is a tradition method of testing protein in reagent strips.

• Protein error of indicators principle is colorimetric reaction.

• Normally in response to specific pH range, indicators produces particular color, in contrary to this, in the constant pH also, indicators change color when protein exist.

• This is due to albumin (protein) which accepts hydrogen ion from indicator.

• Acid buffer is also placed in protein area to maintain pH.

• When concentration of protein increased, a shade from green to blue color appears.

• The readings are read manually or by automated clinitek instruments.

Therefore, “the protein error of indicator” principle is used in the Multistix Pro reagent strip in protein-high pad. Hence, the correct answer is option.