Urinalysis and Body Fluids 6th Edition by Majorie DiLorenzo, Susan Strasinger

Exercise 9

Testing a refrigerated specimen that has not warmed to room temperature will adversely affect:

A. Enzymatic reactions

B. Dye-binding reactions

C. The sodium nitroprusside reaction

D. Diazo reactions

Step 1 of 2

Specimens in the laboratory are refrigerated for preservation purpose.

• Before testing the specimen with reagent strip the specimen must be returned to the room temperature.

• The reagent strips reaction depends on the temperature. If the specimen is not at room temperature and the reagent strip testing is done, it will affect the enzymatic reactions of the strip.

Enzymatic reactions:

• Reaction between the reagent strip chemical and solution which is placed on the reagent strip.

• The solution to be tested contains glucose, proteins, bilirubin etc., and the absorbent pad contains cellulose.

Therefore, “Enzymatic reactions” are affected in the specimen when refrigerated and when not returned to room temperature. Hence, the correct answer is option.