When 10,000 Molecules of ATP Are Hydrolyzed to ADP and Pi

When 10,000 molecules of ATP are hydrolyzed to ADP and Pi in a test tube, about twice as much heat is liberated as when a cell hydrolyzes the same amount of ATP. Which of the following is the best explanation for this observation?

A)Cells are open systems, but a test tube is a closed system.
B)Cells are less efficient at heat production than nonliving systems.
C)The hydrolysis of ATP in a cell produces different chemical products than does the reaction in a test tube.
D)The reaction in cells must be catalyzed by enzymes, but the reaction in a test tube does not need enzymes.
E)Reactant and product concentrations are not the same

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