Quiz 5: Kinetic Analysis of Tyrosinase
In this problem, we are asked to pick which wavelength in a pair has the higher energy: A) 1 nm (X-ray) or 10,000 nm (IR)B) 280 nm (UV) or 360 nm (VIS)C) 200,000 nm (microwave) or 800 nm (VIS)As a rule, the smaller the wavelength, the higher the energy of the wave in question. a)The x-ray is higher energy, as it has the smaller wavelength. b)The UV wave is higher energy, as it has the smaller wavelength. c)The visible light has higher energy, as it has a smaller wavelength.
In this problem, we are asked to calculate the concentration of an unknown solution of compound X. The A 425 of 0.8 and a molar extinction coefficient of 1.5x10 3 M -1 cm -1. The solution has been diluted 1:1 with water. Beer-Lambert governs the movement of light through a fluid that has material dissolved within; the more material is dissolved, the less light will pass through the fluid to the other side and be detected. The formula for calculating this concentration is the Beer-Lambert Law: Where: A the absorbance ? molar extinction coefficient l path length c concentration in M Our calculation for this will be: The calculated concentration is 5.33x10 -4 M; however, this sample was diluted 1:1, so the final concentration is twice this calculated value, or 10.7x10 -4 M.
In this problem, we are asked to match the regions of the electromagnetic spectrum react with a particular transition in a targeted molecule. The spectral regions are x-ray, UV, VIS, IR, and microwave. The transitions in question are inner-shell electrons, molecular rotation, valence electrons, or molecular vibrations. X-rays impart their energy to inner-shell electrons, causing promotions that can be detected by specific equipment. UV rays impart their energy to inner-shell electrons, promotions that are detected by the UV spectrograph. Visible rays impart their energy to valence electrons, causing promotions that can be detected by the naked eye as colors. IR rays impart their energy to molecules, causing an increase in vibrations, which are detected by an IR spectrograph as shifts along certain bonds. Microwaves impart their energy to molecules, causing a change in rotational speed. This can be detected as an increase in temperature, as it causes a change in kinetic energy.
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