## Quiz 3 :

Using Gel Filtration to Study Ligand-Protein Interactions

Answer:

In this problem, we are asked to supply a list of four natural of synthetic molecules that can be transported by albumin.

As a transport protein, it is the job of albumin to bind a variety of molecules and move them from their point of origin (digestive tract, lungs, intravenous introduction, etc) to the location where they will have their action. Albumin does this by creating non-covalent interactions with the material, binding it up, and then moving it through the blood until it reaches the location where it should take effect - removal of the material from albumin is often a matter of diffusion driving dissociation from the albumin into the serum at a new location.

Natural materials that can be transported by albumin include fatty acids, bilirubin, thyroxine, and a number of steroid hormones. Synthetic molecules include aspirin (acetylsalicylic acid), sulfaniliamide compounds, clofibrate, and digitalis - a molecule with cardiac functions.

Answer:

In this problem, we are asked to derive a linear relationship from the following equation: This equation would be used to discussion speed of an enzyme reaction in relation to the point at which an enzyme L reaches half of its saturation point - this term is denoted as K f. If we graph this equation as is, we will receive an unusual curve, which will be hard to relate to other processes and difficult to assess intersections. By taking the reciprocal of this equation and using some algebraic rearrangement, we can turn this equation into a linear representation.

We begin with our equation: We take the reciprocal and end up with: We rearrange, and end up with:

Answer:

In this problem, we are asked to graph the following data on phenol red binding and provide an explanation for the trend. The data above becomes the following graph: As the pH increases, the affinity of phenol red for binding decreases; therefore, phenol red's binding is pH-dependent.

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