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Concepts of Genetics 11th Edition by William Klug,Michael Cummings,Charlotte Spencer,Michael Palladino
Edition 11ISBN: 9781292139456Concepts of Genetics 11th Edition by William Klug,Michael Cummings,Charlotte Spencer,Michael Palladino
Edition 11ISBN: 9781292139456 Exercise 30
A widely used method for calculating the annealing temperature for a primer used in PCR is 5 degrees below the T m (°C), which is computed by the equation 81.5 + 0.41 × (%GC) - (675/ N ), where %GC is the percentage of GC nucleotides in the oligonucleotide and N is the length of the oligonucleotide. Notice from the formula that both the GC content and the length of the oligonucleotide are variables. Assuming you have the following oligonucleotide as a primer, compute the annealing temperature for PCR. What is the relationship between T m (°C) and %GC? Why? ( Note : In reality, this computation provides only a starting point for empirical determination of the most useful annealing temperature.)5?-TTGAAAATATTTCCCATTGCC-3?
Explanation
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The T m (melting temperature) in degrees...
Concepts of Genetics 11th Edition by William Klug,Michael Cummings,Charlotte Spencer,Michael Palladino
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