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Concepts of Genetics 11th Edition by William Klug,Michael Cummings,Charlotte Spencer,Michael Palladino
Edition 11ISBN: 9781292139456Concepts of Genetics 11th Edition by William Klug,Michael Cummings,Charlotte Spencer,Michael Palladino
Edition 11ISBN: 9781292139456 Exercise 15
You have recovered a cloned DNA segment from a vector and determine that the insert is 1300 bp in length. To characterize this cloned segment, you isolate the insert and decide to construct a restriction map. Using enzyme I and enzyme II, followed by gel electrophoresis, you determine the number and size of the fragments produced by enzymes I and II alone and in combination, as recorded in the following table. Construct a restriction map from these data, showing the positions of the restriction-enzyme cutting sites relative to one another and the distance between them in units of base pairs.
Explanation
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We first draw restriction sites for the ...
Concepts of Genetics 11th Edition by William Klug,Michael Cummings,Charlotte Spencer,Michael Palladino
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