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Concepts of Genetics 11th Edition by William Klug,Michael Cummings,Charlotte Spencer,Michael Palladino
Edition 11ISBN: 9781292139456Concepts of Genetics 11th Edition by William Klug,Michael Cummings,Charlotte Spencer,Michael Palladino
Edition 11ISBN: 9781292139456 Exercise 24
A fellow student considers the issues in Problem and argues that there is a more straightforward, nongenetic experiment that could differentiate between the two types of mutations. The experiment requires no fancy genetics and would allow you to easily assay the products of the other SOS genes. Propose such an experiment.
The SOS repair genes in E. coli (discussed in Chapter 15) are negatively regulated by the lexA gene product, called the LexA repressor. When a cell sustains extensive damage to its DNA, the LexA repressor is inactivated by the recA gene product (RecA), and transcription of the SOS genes is increased dramatically.
One of the SOS genes is the uvrA gene. You are a student studying the function of the uvrA gene product in DNA repair. You isolate a mutant strain that shows constitutive expression of the UvrA protein. Naming this mutant strain uvrA C , you construct the following diagram showing the lexA and uvrA operons:
(a) Describe two different mutations that would result in a uvrA constitutive phenotype. Indicate the actual genotypes involved.
(b) Outline a series of genetic experiments that would use partial diploid strains to determine which of the two possible mutations you have isolated.
The SOS repair genes in E. coli (discussed in Chapter 15) are negatively regulated by the lexA gene product, called the LexA repressor. When a cell sustains extensive damage to its DNA, the LexA repressor is inactivated by the recA gene product (RecA), and transcription of the SOS genes is increased dramatically.
One of the SOS genes is the uvrA gene. You are a student studying the function of the uvrA gene product in DNA repair. You isolate a mutant strain that shows constitutive expression of the UvrA protein. Naming this mutant strain uvrA C , you construct the following diagram showing the lexA and uvrA operons:
(a) Describe two different mutations that would result in a uvrA constitutive phenotype. Indicate the actual genotypes involved.
(b) Outline a series of genetic experiments that would use partial diploid strains to determine which of the two possible mutations you have isolated.
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An assay could be developed that analyze...
Concepts of Genetics 11th Edition by William Klug,Michael Cummings,Charlotte Spencer,Michael Palladino
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