Deck 4: Care and Use of the Microscope

Question 1

Where should the condenser be located when using the *100 oil objective?

A)lowest position
B)highest position
C)middle position
D)variable depending on amount of light desired

Answer 1

highest position

Question 2

What is the most likely reason for cells on a hemocytometer appearing "fuzzy" when switching from low power to the *40 objective?

A)condenser not lowered enough
B)hemocytometer not cleaned properly
C)oil on the 40* lens
D)cells not allowed to settle

Answer 2

oil on the 40* lens

Question 3

An object is brought into focus using the 10* objective lens.When the 40* objective lens is moved into position,only a minor adjustment using the fine focus adjustment is required to bring it clearly into focus.What is the term used to describe lenses with this feature?

A)parcentric
B)achromatic
C)plan-achromatic
D)parfocal

Answer 3

parfocal

Question 4

What solution is best for cleaning lenses?

A)commercial glass-cleaning solution (e.g., Windex)
B)water
C)benzene
D)xylol

Question 5

Which of the following would be least likely to cause a "fuzzy" image on oil power?

A)using a plan-achromatic objective lens
B)objective not screwed in tightly
C)not enough oil on the slide when using oil immersion lens
D)focus controls not adjusted

Question 6

The ability to distinguish between two objects that are situated close to one another as separate entities is

A)resolution.
B)parfocal.
C)contrast.
D)parcentric.

Question 7

How should a microscope be carried?

A)both hands supporting the base
B)both hands around the arm
C)one hand around the arm and the other holding the oculars in place
D)one hand under the base and the other around the arm

Question 8

Why is it not advisable to interchange one ocular lens with another from a different brand microscope?

A)The two ocular lenses in a microscope are optically matched with one another.
B)The fitting for the ocular lenses varies, so they will not fit in another microscope.
C)They cannot be removed from the microscope.
D)They are fragile and are likely to break when removed from the microscope.

Question 9

The device used to assist in finding a cell of interest at a later time is called a

A)microlocator.
B)micrometer.
C)reticle.
D)hemocytometer.

Question 10

What immersion oil is used most commonly in hematology?

A)type A
B)type B
C)type C
D)type D

Question 11

What should be adjusted if two overlapping images,instead of only one,are seen when looking through a microscope?

A)field diaphragm
B)fine adjustment knob
C)condenser knob
D)interpupillary control

Question 12

An image viewed under the microscope is clearly seen under both the 10* and 40* objectives,but it is not visible under *100.What is the most likely reason for this?

A)Oil is too thick on the slide.
B)The slide is placed upside down on the stage.
C)The slide has a coverslip on it.
D)No oil is on the slide.

Question 13

What is the purpose of the blue filter when using a tungsten-halogen microscope light?

A)eliminate blue color
B)eliminate yellow color
C)reduce light intensity
D)filter out stray light

Question 14

What is the final magnification of an image if the ocular lens is 15* and the objective lens is 50*?

A)*150
B)*500
C)*750
D)*1000

Question 15

Match each lettered type of microscope to its clinical use.

Used with fluorochrome dyes to identify lymphocyte subsets

A)Phase contrast
B)Darkfield
C)Polarized light
D)Fluorescent
E)Brightfield

Question 16

Which of the following microscopic procedures requires high contrast and lower light?

A)examining unstained objects (e.g., urine sediments)
B)evaluating red cell morphology
C)manual differentials
D)platelet estimates

Question 17

Koehler illumination

A)is best achieved using a tungsten-halogen light bulb.
B)allows the brightest amount of light to be in the center of a field.
C)adjusts for even light across a field.
D)changes the intensity of the light across a field.

Question 18

What can increase the resolution of an object under the microscope?

A)lowering the condenser
B)increasing the combined magnification provided by the ocular and objective lenses
C)enlarging the numeric aperture of the objective lens
D)adjusting the focus controls

Question 19

What converts for curvature in a field so that a flat field with uniform focus is seen?

A)achromatic objective lens
B)plan-achromatic objective lens
C)spherical correction ocular lens
D)blue filter when placed above the condenser

Question 20

What type of microscope helps in the identification of urine crystals?

A)compound brightfield
B)phase contrast
C)polarized light
D)darkfield

Question 21

Match each lettered type of microscope to its clinical use.

Identifying crystals in synovial fluid

A)Phase contrast
B)Darkfield
C)Polarized light
D)Fluorescent
E)Brightfield

Question 22

Match each lettered type of microscope to its clinical use.

Counting platelets manually

A)Phase contrast
B)Darkfield
C)Polarized light
D)Fluorescent
E)Brightfield

Question 23

Match each lettered type of microscope to its clinical use.

Routine differentials

A)Phase contrast
B)Darkfield
C)Polarized light
D)Fluorescent
E)Brightfield

Question 24

Match each lettered type of microscope to its clinical use.

Viewing spirochetes in microbiology

A)Phase contrast
B)Darkfield
C)Polarized light
D)Fluorescent
E)Brightfield