Biochemistry 6th Edition by Reginald Garrett,Charles Grisham

Exercise 25

Exploring the Mechanism of lipid Transport by a Flippase ATP-dependent flippases are closely related in their structures to ion pumps like the plasma membrane Na + , K + -ATPase and sarcoplasmic reticulum Ca 2+ -ATPase, but they contend with a novel dilemma not shared by the ion transport systems. Consult the following reference and summarize the structural features common to the flippases and P-type ATPases and describe how the flippase solves the "giant substrate problem". (Vestegaard, A.L., Coleman, J.A., Lemmin, T., et al., 2014. Critical roles of isolcucine-364 and adjacent residues in a hydrophobic gate control of phospholipid transport by the mammalian P4-ATPase ATP8A2. Proceedings the National Academy of Sciences USA 111 :16629-16636.)