Biology 3rd Edition by Peter Russell, Paul Hertz, Beverly McMillan

Exercise 1

You have a culture of Hfr E.coli cells that cannot make biotin for themselves.To this culture you add some wild-type E.coli cells that have been heat killed, and then subject the culture to electroporation.After the addition, you find some cells that can grow on minimal medium.How could you establish whether the wild-type bio allele was inserted in a plasmid or the chromosomal DNA of the Hfr cells?