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Molecular Biology 5th Edition by Robert Weaver
Edition 5ISBN: 978-0073525327Molecular Biology 5th Edition by Robert Weaver
Edition 5ISBN: 978-0073525327 Exercise 2
The following is a physical map of a region you are mapping by RFLP analysis. 
The numbered vertical lines represent restriction sites recognized by Sma I. The circled sites (2 and 3) are polymorphic, the others are not. You cut the DNA with Sma I , electrophorese the fragments, blot them to a membrane, and probe with a DNA whose extent is shown at top. Give the sizes of fragments you will detect in individuals homozygous for the following haplotypes with respect to sites 2 and 3.
The numbered vertical lines represent restriction sites recognized by Sma I. The circled sites (2 and 3) are polymorphic, the others are not. You cut the DNA with Sma I , electrophorese the fragments, blot them to a membrane, and probe with a DNA whose extent is shown at top. Give the sizes of fragments you will detect in individuals homozygous for the following haplotypes with respect to sites 2 and 3.
Explanation
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The RFLP is an abbreviation for " Restri...
Molecular Biology 5th Edition by Robert Weaver
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