Molecular Biology 5th Edition by Robert Weaver

Exercise 2

You are investigating the pot1 gene of a newly-discovered protozoan species. You fi nd that cells with a defective pot1 gene return to normal after 50 generations. Wildtype cells have only two chromosomes with the following restriction maps with respect to the restriction enzyme Zap I:
Propose a hypothesis to explain how the mutant cells returned to normal, and describe an experiment you would perform to test it. Show the results you would obtain if your hypothesis is correct.