Molecular Biology 5th Edition by Robert Weaver

Exercise 1

Transcription of a class II gene starts at a guanosine 25 bp downstream of the last base of the TATA box. You delete 20 bp of DNA between this guanosine and the TATA box and transfect cells with this mutated DNA. Will transcription still start at the same guanosine If not, where How would you locate the transcription start site