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Molecular Biology 5th Edition by Robert Weaver
Edition 5ISBN: 978-0073525327Molecular Biology 5th Edition by Robert Weaver
Edition 5ISBN: 978-0073525327 Exercise 4
You are studying a new operon in E. coli involved in phenylalanine biosynthesis.
a. How would you predict this operon is regulated (inducible or repressible by phenylalanine, positive or negative) Why
b. You sequence the operon and discover that it contains a short open reading frame near the 5-end of the operon that contains several codons for phenylalanine. What prediction would you make about this leader sequence and the peptide that it encodes
c. What would happen if the sequence of this leader were changed so that the phenylalanine codons (UUU, UUU) were changed to leucine codons (UUA, UUG)
d. What is this kind of regulation called and would it work in a eukaryotic cell Why or why not
a. How would you predict this operon is regulated (inducible or repressible by phenylalanine, positive or negative) Why
b. You sequence the operon and discover that it contains a short open reading frame near the 5-end of the operon that contains several codons for phenylalanine. What prediction would you make about this leader sequence and the peptide that it encodes
c. What would happen if the sequence of this leader were changed so that the phenylalanine codons (UUU, UUU) were changed to leucine codons (UUA, UUG)
d. What is this kind of regulation called and would it work in a eukaryotic cell Why or why not
Explanation
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Verified
a) Since this operon regulates the gene'...
Molecular Biology 5th Edition by Robert Weaver
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