Genetics: Analysis and Principles 4th Edition by Robert Brooker

Exercise 8

In the experiment of Figure 14.7, a lacI - mutant was conjugated to a strain that had a functional lacI gene on an F factor. The results of this experiment were important in determining the action of the lac repressor protein. What results would you expect if you used the same approach to investigate the regulation of the ara operon via AraC In other words, what would be the level of expression of the ara operon in an araC - strain in the presence and absence of arabinose, and how would the level of expression change when a functional araC gene was introduced into the strain via conjugation