Urinalysis and Body Fluids 6th Edition by Majorie DiLorenzo, Susan Strasinger

Exercise 31

Discuss the collection procedure for a quantitative fecal fat and methods for analysis.

Step 1 of 5

Collection procedure for quantitative fecal fat:

• Timed specimen is needed for quantitative fecal fat

• To test the fecal fat, the stool should be collected for 3 days and the samples are collected in a large, pre-weighed container

• The patient should intake 100 grams of fat per day before and during collection of specimen

• The specimen should not be contaminated with urine or water and it should be sealed and taken immediately to the laboratory

• Prior to analysis, the specimen is weighed and homogenized

• Refrigeration of specimen prevents bacterial degradation

• The specimen within the container should be opened carefully and the outside of the container should not be contaminated

• Small sample is collected after the feces in container are thoroughly mixed

• The fat is extracted with the solvent and measured with saponification