Urinalysis and Body Fluids 6th Edition by Majorie DiLorenzo, Susan Strasinger

Exercise 34

When performing a sperm concentration, 60 sperm are counted in the RBC squares on one side of the emocytometer

and 90 sperm are counted in the RBC squares on the other side. The specimen is diluted 1:20. The:

A. Specimen should be rediluted and counted

B. Sperm count is 75 million/mL

C. Sperm count is greater than 5 million/mL

D. Sperm concentration is abnormal

Step 1 of 2

Sperm counting process:

Semen analysis is a process of evaluating male fertility through microscopic and macroscopic examination.

• In the laboratory, sperm counting process is done using Neubauer hemocytometer.

• Four squares at the corners and one large square at the center is for counting the sperms and allowed 3 to 5 minutes to settle down.

• Both side of hemocytometer is loaded with sperms(each side gives individual count)

• Then the counting of sperm are started, the average of two counts should not be greater than 10%.

• If the count exceeds the difference of 10%, then the semen specimen should be diluted and counted again.

• When, 60 sperms are counted in one side of RBC squares and 90 sperms are counted in other side of the RBC squares of hemocytometer.

• Total number of sperms present in the RBC squares is “150”. To find its average it should be divided by number of squares which is “2”.

• 75% is not average. The count and dilution of sperm should be repeated.

Therefore, the difference between two squares is more than 10%; hence, the semen specimen should be diluted and counted again. Hence, the correct answer is option.