Urinalysis and Body Fluids 6th Edition by Majorie DiLorenzo, Susan Strasinger

Exercise 66

A speckled pattern on the blood pad of the reagent strip indicates:

A. Hematuria

B. Hemoglobinuria

C. Myoglobinuria

D. All of the above

Step 1 of 2

Reagent strip reaction of blood:

Reagent strip reaction for blood is performed to detect blood in urine.

• The principle is “heme peroxidase” activity.

Procedure:

• The reagent strip has peroxide and tetramethylbenzidine in testing area for blood.

• Hemoglobin and myoglobin’s heme component reacts with tetramethylbenzidine (chromogen) to give oxidized chromogen in the color of green-blue.

Myoglobin:

Oxygen and iron binding protein in muscle tissue.

• Color range from negative yellow ->green->positive green->blue are formed in the occurrence of free hemoglobin or myoglobin in urine.

• In contrast, when red blood cells are destroyed and contacts the pad, released hemoglobin forms a reaction which gives speckled configuration on pad.

• This speckled pattern indicates the presence of hematuria.

Hematuria:

• Existence of red blood cells (blood) in urine caused by glomerular diseases ,renal calculi, tumors, toxic chemical exposure, anti-coagulant therapy and trauma.

Therefore, “hematuria” presence causes speckled pattern on reagent strip for blood. Hence, the correct answer is option.