Urinalysis and Body Fluids 6th Edition by Majorie DiLorenzo, Susan Strasinger

Exercise 53

The principle of the reagent strip tests for glucose is the:

A. Peroxidase activity of glucose

B. Glucose oxidase reaction

C. Double sequential enzyme reaction

D. Dye-binding of glucose and chromogen

Step 1 of 2

Glucose reagent strip test:

Presence of glucose in urine is detected by this test.

• The principle is “double sequential enzyme reaction”.

Procedure:

• The test area is provided with glucose oxidase, chromogen, peroxidase and buffer which give double sequential enzyme reaction.

Step 1:

• Glucose in urine and air (in the room) reacts by glucose oxidase to give peroxide and gluconic acid.

Step 2:

• Peroxide and chromogen reacts by peroxidase to give oxidized colored chromogen.

• As this reaction is in sequence exhibited by enzyme action it is called double sequence enzyme reaction.

• Potassium iodide (green->brown) is a chromogen of Multistix brand.

• Tetramethylbenzidine (yellow->green) is a chromogen brand of chemstrip.

• Result reported as 1+,2+,3+,4+ glucose and also color charts provide or 0.1% to 2% or 100 mg/dL (milligram per deciliter) to 2 g/dL (gram per deciliter) (quantitative measurement).

Therefore, “double sequential enzyme reaction” is the principle of glucose reagent strip test. Hence, the correct answer is option.