Urinalysis and Body Fluids 6th Edition by Majorie DiLorenzo, Susan Strasinger

Exercise 37

The primary chemical on the reagent strip in the Micral-Test for microalbumin binds to:

A. Protein

B. Antihuman albumin antibody

C. Conjugated enzyme

D. Galactoside

Step 1 of 2

Micral-test:

• It is a test to detect albumin in reagent strip.

• It also measures creatinine to give albumin creatinine ratio in reagent strip.

• It is an immunochemical assay test.

• Micro albuminuria test is to detect presence of albumin in urine.

Immuno chemical assay:

• It is a biochemical test.

• Using immunoglobulin or antibody, concentration of a macromolecule is measured in a solution.

Types of Micral test:

• Roche diagnostics

• Indianapolis

• IN

Test procedure:

• A gold labeled antibody-enzyme conjugate contains anti human albumin in Micral test reagent strip.

• The strips are dipped in urine up to the marked level present on the strip for a time of 5 seconds.

• Urine albumin combines with the antibody.

• In captive zone, unbound conjugates are cleared by joining with the albumin in strip.

• Albumin bounded with urine conjugates, reach the enzyme substrate in strip thus producing colors from white to red by the action of substrate with the conjugated enzyme.

Therefore, “antihuman albumin antibody” binds to the primary chemical used in Micral test for microalbumin. Hence, the correct answer is option.